Application de la microfluidique de gouttes à la conception d’un appareil de PCR en flux continu

Using droplet microfluidics to design a continuous flow PCR system

Institut Curie, Laboratoire de Physico-Chimie, 11 rue Pierre et Marie Curie, 75005 Paris

Auteur de correspondance : max.chabert@curie.fr

Abstract

The design of a two-phase-flow (water in oil) microfluidic PCR system led us to solve different problems in order to achieve DNA amplification : droplet train instability, cross contamination between droplets, being able to coalesce two droplets without direct contact with them. After a short explanation of the PCR principle, the experimental conditions used to achieve droplets train stability and avoid contamination between droplets will be presented, as well as the amplification results obtained. We will then present a system based on the electrocoalescence principle which allows to coalesce without direct contact two water droplets inside the capillary.