| Issue |
La Houille Blanche
Number 5, Octobre 2003
|
|
|---|---|---|
| Page(s) | 24 - 28 | |
| DOI | https://doi.org/10.1051/lhb/2003085 | |
| Published online | 01 July 2009 | |
Un dispositif d'écoulement pour l'étude des réactions cellulaires au contact d'une paroi poreuse
A flow device to study the responses of cells adhering to a porous wall
1
UMR CNRS 6600 " Biomécanique et Génie Biomédical ", Université de Technologie de Compiègne
2
UMR CNRS 5520 - UJF-INP Grenoble
3
Biophysique et Imagerie Médicale UPJV Amiens
Auteurs de correspondance : Agnes.Drochon@utc.fr Roxana.Ghodsnia@ujf-grenoble.fr
Abstract
Biomaterials used in biomedical devices, such as bioartificial organs, hemodialysers and vascular prosthesis, are porous and are exposed to normal and tangential flow of physiological fluid. Flow induced forces may influence the responses (morphology, migration, detachment) of cells adhering to a porous wall. In classical in-vitro flow devices, cells are submitted to only tangential stresses. We designed a new flow system in order to take into account the influence of a transmural pressure. It is a parallel-plate flow chamber with a porous bottom wall (biomaterial). We report an application of this system to study the effect of flow conditions on cells adhering to a dialysis membrane. The mechanically induced cell shape changes are correlated to biochemical analysis (activation of the cAMP pathway).
© Société Hydrotechnique de France, 2003