La Houille Blanche
Number 5, Octobre-Novembre 2006
|Page(s)||51 - 56|
|Published online||01 June 2007|
Application de la microfluidique de gouttes à la conception d’un appareil de PCR en flux continu
Using droplet microfluidics to design a continuous flow PCR system
Institut Curie, Laboratoire de Physico-Chimie, 11 rue Pierre et Marie Curie, 75005 Paris
Auteur de correspondance : firstname.lastname@example.org
The design of a two-phase-flow (water in oil) microfluidic PCR system led us to solve different problems in order to achieve DNA amplification : droplet train instability, cross contamination between droplets, being able to coalesce two droplets without direct contact with them. After a short explanation of the PCR principle, the experimental conditions used to achieve droplets train stability and avoid contamination between droplets will be presented, as well as the amplification results obtained. We will then present a system based on the electrocoalescence principle which allows to coalesce without direct contact two water droplets inside the capillary.
Mots clés : Congrès microfluidique 2004
© Société Hydrotechnique de France, 2006
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