La Houille Blanche
Number 6, Décembre 2007
|Page(s)||34 - 38|
|Section||Microfluidique 2006 : Visualisation et mesures|
|Published online||03 January 2008|
Autoassemblage de colloïdes magnétiques sur un réseau de plots en nickel : Application à l’électrophorèse de longs ADN
Magnetic beads self-organization on ni pattern : Application to long DNA electrophoresis
Institut Curie, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris, France
2 Laboratoire de Photonique et Nanostructures, LPN-CNRS, route de Nozay, 91460 Marcoussis, France
Auteur de correspondance : firstname.lastname@example.org
When placed in a Hele-Shaw cell and submitted to a uniform magnetic field, a suspension of superparamagnetic particles self-organizes into a 2D array of columns with hexagonal order on short distances and glasslike order on long distances. Such arrays were usefully used for fast electrophoretic separation of long DNA molecules. Although self-assembled matrices have the advantages of simplicity and tunability, it is impossible to have the precisely controlled order and pore size obtainable in a microlithographic array. We address the latter challenge here. A magnetic nickel template is prepared on one cell surface by lift-off. Under a uniform external field, the nickel dots create local magnetic field gradients that attracts the beads, imposing a predefined order. We demonstrate the ability to separate long DNA molecules with these matrices and achieve the first steps of studying the resolution of the DNA separation over a wide range of posts density.
© Société Hydrotechnique de France, 2007
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