| Issue |
La Houille Blanche
Number 4, Août 2003
|
|
|---|---|---|
| Page(s) | 31 - 36 | |
| DOI | https://doi.org/10.1051/lhb/2003072 | |
| Published online | 01 July 2009 | |
Microsystème fluidique d'analyse proteomique par ESI-MS
Microfluidic system for proteomics analysis by ESI-MS
1
USTL, UPRESA CNRS 8009
2
IEMN, UMR CNRS 8520
Abstract
We report here on the fabrication of a novel silicon microfluidic system dedicated to protein sample preparation prior to their on-line analysis by electrospray and mass spectrometry (ESI-MS). The microsystem includes several main module, such as a digestion cell, an elution column, a pumping system as well as an integrated nano-ESI source, whose realization reflects our technological choice. After a brief presentation on microsystems, we describe our work in microfluidics. the conception and the description of the different modules, the accounting for the retained technological alternatives and the first realizations.
© Société Hydrotechnique de France, 2003