Microsystème fluidique d'analyse proteomique par ESI-MS

Microfluidic system for proteomics analysis by ESI-MS

¹ USTL, UPRESA CNRS 8009
² IEMN, UMR CNRS 8520

Abstract

We report here on the fabrication of a novel silicon microfluidic system dedicated to protein sample preparation prior to their on-line analysis by electrospray and mass spectrometry (ESI-MS). The microsystem includes several main module, such as a digestion cell, an elution column, a pumping system as well as an integrated nano-ESI source, whose realization reflects our technological choice. After a brief presentation on microsystems, we describe our work in microfluidics. the conception and the description of the different modules, the accounting for the retained technological alternatives and the first realizations.